Plasmid

Part:BBa_K4876006

Designed by: Jin Luming   Group: iGEM23_Zhejiang-United   (2023-09-28)


colE1-cas9-op-λ-Red

colE1-cas9-op-λ-Red

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 157
    Illegal NheI site found at 2638
    Illegal NheI site found at 11382
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 15087
    Illegal BglII site found at 15546
    Illegal BglII site found at 17475
    Illegal BamHI site found at 4914
    Illegal BamHI site found at 5774
    Illegal BamHI site found at 17277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5800
    Illegal NgoMIV site found at 10479
    Illegal NgoMIV site found at 11388
    Illegal NgoMIV site found at 17548
    Illegal AgeI site found at 243
    Illegal AgeI site found at 567
    Illegal AgeI site found at 6123
    Illegal AgeI site found at 13240
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 14068
    Illegal SapI site found at 11507
    Illegal SapI.rc site found at 8898
    Illegal SapI.rc site found at 9140
    Illegal SapI.rc site found at 16968


[edit]
Categories
Parameters
None