Plasmid_Backbone

Part:BBa_K4875024

Designed by: Cheng Ziyan   Group: iGEM23_Metropolis-Shanghai   (2023-08-24)


pVenus-backbone

pVenus-backbone


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 613
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 644
    Illegal BamHI site found at 749
    Illegal XhoI site found at 648
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 1331
    Illegal NgoMIV site found at 2614
    Illegal NgoMIV site found at 2897
    Illegal AgeI site found at 622
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 3109
    Illegal SapI.rc site found at 2463
    Illegal SapI.rc site found at 2673


[edit]
Categories
Parameters
None