Part:BBa_K4869006

Terminal inverted repeat sequence

General Information & Literature Review:

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Figure: Schematic representation of terminal inverted repeat sequence.

Terminal inverted repeat (TIR) sequences are essential for the transposition of most transposons. They are distinct DNA structures found at the ends of certain transposable elements (TEs). TIR sequences are substantially similar and are made of two sequences that are inverted complements of each other, meaning that the complementary strand would read right to left in one strand of the TIR was read from left to right. Typically, the inverted repetitions range in length from a few to several hundred base pairs.

For the transposition of many different forms of transposable elements, notably DNA transposons, TIR sequences are crucial. The transposase enzyme, which is in charge of slicing and pasting the transposon into a new position in the genome, uses them as recognition sites. The excision and integration of the transposon are facilitated by the transposase enzyme, which identifies and binds to the TIR sequences.

Application in Our Project:

In experimental contexts, TIR sequences are essential in utilizing the maneuverability of DNA transposons and certain retrotransposons, facilitating precise genetic insertions via a "cut and paste" methodology. Particularly, the piggyBac transposon system, utilizing TIR sequences, enables scientists to strategically insert genes into a host genome, such as HEK293 genomic DNA, by employing a transposase enzyme. This system proves invaluable in gene therapy and genetic engineering experiments by allowing for the meticulous and targeted insertion of therapeutic or desired genes, thereby enhancing the efficiency and accuracy of genetic manipulations and interventions within research and biotechnological applications.

References:

“Inverted Terminal Repeat.” Inverted Terminal Repeat - an Overview | ScienceDirect Topics, www.sciencedirect.com/topics/neuroscience/inverted-terminal-repeat. Accessed 4 Oct. 2023.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]