Part:BBa_K4869005
Homo sapience (human) Wnt3a gene
General Information & Literature Review:
Homo sapiens (human) Wnt3a gene was used in this study (1059 bp). Wnt3a is a member of the Wnt family of secreted signaling proteins that play a crucial role in cell proliferation and other cellular processes. Wnt3a activates the canonical Wnt signaling pathway, which regulates the transcription of various genes involved in cell proliferation, differentiation, and survival. Wnt3a has been shown to play a critical role in the proliferation of stem cells, as well as the maintenance of adult tissues such as the intestine, skin, and hair follicles. Studies have also suggested that aberrant Wnt3a signaling can contribute to the development and progression of various types of cancer, as dysregulated Wnt signaling can promote uncontrolled cell proliferation and tumor growth. Wnt3a is a key ligand of the canonical Wnt/β-catenin pathway and supports strongly increased self-renewal of organ and embryonic stem cells and the serum-free establishment of human organoids from healthy and diseased livers.
The in-depth exploration of the Wnt3a gene sequencing data may be facilitated through rigorous examination of online databases, notably GenBank, Ensembl, and the UCSC Genome Browser. These databases not only proffer sequence information but also offer annotations that are pivotal for comprehending the genomic organization of the gene, incorporating both exons (coding segments) and introns (non-coding segments).
Wnt3a, in its role as a ligand, engages with Frizzled receptors on cellular surfaces, thereby precipitating a multitude of intracellular signaling pathways, notably the canonical β-catenin-dependent pathway. This mechanism proves fundamental in the regulation of cellular fate and in bolstering embryonic development. Moreover, aberrations in the expression or functionality of Wnt3a have been associated with a diverse array of pathologies, including cancers, due to its salient role in regulating cellular proliferation and differentiation. Therefore, the utilization of scientific databases and literature, which go through regular updates and peer review, is paramount to secure accurate information regarding the sequence and attributes of the Wnt3a gene.
Application in Our Project:
Experimentally, the Wnt3a gene has been substantiated to enhance the proliferation of bovine muscle cells. By incorporating Wnt3a in conditioned media, there is potential to amplify the proliferation and differentiation of bovine muscle cells, thereby offering a pathway toward a more economical and sustainable methodology for beef production. Furthermore, Wnt signaling has the aptitude to stimulate the proliferation of muscle stem cells, known as satellite cells, which are indispensable in the production of cultured meat. The activation of the Wnt pathway can induce satellite cells to divide and differentiate into mature muscle cells, which can subsequently be harvested and processed into meat products while upholding principles of sustainability and economic viability.
References:
Benny, A., Pandi, K., & Upadhyay, R. (2022, July 20). Techniques, challenges and future prospects for cell-based meat. Food science and biotechnology. www.ncbi.nlm.nih.gov/pmc/articles/PMC9385919/.
Shang, Y., Wang, S., Xiong, F., Zhao, C., Peng, F., Feng, S., Li, M., Li, Y., & Zhang, C. (n.d.). WNT3A signaling promotes proliferation, myogenic differentiation, and migration of rat bone marrow mesenchymal stem cells. Nature News. www.nature.com/articles/aps2007216.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 30
Illegal PstI site found at 165
Illegal PstI site found at 411 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 30
Illegal PstI site found at 165
Illegal PstI site found at 411
Illegal NotI site found at 245
Illegal NotI site found at 768
Illegal NotI site found at 935 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 30
Illegal PstI site found at 165
Illegal PstI site found at 411 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 30
Illegal PstI site found at 165
Illegal PstI site found at 411 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 760
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