Regulatory

Part:BBa_K4868000

Designed by: Viktor Manuel Grunddal Larsen   Group: iGEM23_SDU-Denmark   (2023-07-24)


Fluoride Riboswitch

The part is a fluoride-dependent riboswitch. Upon binding of fluoride it will allow translation of the downstream genetic elements. The riboswitch includes a ribosomal binding site (RBS), and should be implemented with a short base seperation (~8 bases) from the downstream gene. A promoter is to be included upstream from the riboswitch. The effect of using the riboswtich is a fluoride- and fluoride concentration-dependent translation of any genetic element.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None