Part:BBa_K4849039

MtrCAB + CymA - LvT

Level T expression construct containing MtrCAB and CymA under control of Ptrc10 constitutive Synechocystis sp. And E. coli promoter and TrrnB double terminator.

CymA is involved in shuttling electrons from the inner membrane to the periplasmic space, facilitating the transfer of electrons to these different electron

Metal-reducing microorganisms such as Shewanella oneidensis MR-1 and Geobacter metallireducens have evolved an innate EET pathway that makes them ideal for bioelectricity applications. The most characterised EET pathway in S. oneidensis MR-1 includes MtrCAB complex. Based on the current understanding, the electrons extracted from the oxidation of a substrate are ultimately transferred to the periplasmic decaheme cytochrome MtrA during anaerobic respiration. Subsequent electron transfer to the outer membrane β-barrel protein MtrB places MtrA in direct contact with MtrC. MtrC is re-oxidised by reducing terminal electron acceptors, such as electrodes, via direct or mediated electron transfer.

Image made in Biorender.com References: 1. Ross, Daniel E., et al. "Characterization of protein-protein interactions involved in iron reduction by Shewanella oneidensis MR-1." Applied and environmental microbiology 73.18 (2007): 5797-5808. 2. Breuer, Marian, et al. "Multi-haem cytochromes in Shewanella oneidensis MR-1: structures, functions and opportunities." Journal of The Royal Society Interface 12.102 (2015): 20141117. Sequence and Features BBa_K4849039 SequenceAndFeatures