Part:BBa_K4849037
MtrAB - LvT - cytochrome protein complex
Level T expression construct containing MtrAB under control of Ptrc10 constitutive Synechocystis sp. And E. coli promoter and TrrnB double terminator.
Metal-reducing microorganisms such as Shewanella oneidensis MR-1 and Geobacter metallireducens have evolved an innate EET pathway that makes them ideal for bioelectricity applications. The most characterised EET pathway in S. oneidensis MR-1 includes MtrCAB complex. Based on the current understanding, the electrons extracted from the oxidation of a substrate are ultimately transferred to the periplasmic decaheme cytochrome MtrA during anaerobic respiration. Subsequent electron transfer to the outer membrane β-barrel protein MtrB places MtrA in direct contact with MtrC. MtrC is re-oxidised by reducing terminal electron acceptors, such as electrodes, via direct or mediated electron transfer.
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References:
1. Ross, Daniel E., et al. "Characterization of protein-protein interactions involved in iron reduction by Shewanella oneidensis MR-1." Applied and environmental microbiology 73.18 (2007): 5797-5808.
2. Breuer, Marian, et al. "Multi-haem cytochromes in Shewanella oneidensis MR-1: structures, functions and opportunities." Journal of The Royal Society Interface 12.102 (2015): 20141117.
Sequence and Features
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