Composite

Part:BBa_K4849031

Designed by: Devansh Kumar   Group: iGEM23_Edinburgh   (2023-10-12)


MtrA - LvT - periplasmic cytochrome protein

Level T expression construct containing MtrA under control of Ptrc10 constitutive Synechocystis sp. And E. coli promoter and TrrnB double terminator.


Metal-reducing microorganisms such as Shewanella oneidensis MR-1 and Geobacter metallireducens have evolved an innate EET pathway that makes them ideal for bioelectricity applications. The most characterised EET pathway in S. oneidensis MR-1 includes MtrCAB complex. Based on the current understanding, the electrons extracted from the oxidation of a substrate are ultimately transferred to the periplasmic decaheme cytochrome MtrA during anaerobic respiration. Subsequent electron transfer to the outer membrane β-barrel protein MtrB places MtrA in direct contact with MtrC. MtrC is re-oxidised by reducing terminal electron acceptors, such as electrodes, via direct or mediated electron transfer.

Image made in Biorender.com References: 1. Ross, Daniel E., et al. "Characterization of protein-protein interactions involved in iron reduction by Shewanella oneidensis MR-1." Applied and environmental microbiology 73.18 (2007): 5797-5808. 2. Breuer, Marian, et al. "Multi-haem cytochromes in Shewanella oneidensis MR-1: structures, functions and opportunities." Journal of The Royal Society Interface 12.102 (2015): 20141117. Sequence and Features BBa_K4849031 SequenceAndFeatures

[edit]
Categories
Parameters
None