Coding

Part:BBa_K4843001

Designed by: Wu Delu   Group: iGEM23_TSB-Center   (2023-08-07)


ASFV-I7L gene sequence

Basic Part: BBa_K4843001 (ASFV-i7L)

Basic Part: BBa_K4843001 (ASFV-i7L)

Profile

  • Name: ASFV-i7L
  • Base Pairs: 720 bp
  • Origin: African swine fever virus
  • Properties: The viral protease I7L, which is responsible for the cleavage of A17, a structural protein involved in the formation of the crescent membrane that eventually leads to the virion assembly.

Usage and Biology

According to the literature, within the replication cycle of the model vaccinia virus, a fundamental step is the maturation of non-infective virions from immature intracellular virions (IV) to mature intracellular virions (IMV). This maturation process is linked to the activity of the viral protease I7L, which is responsible for the cleavage of A17, a structural protein involved in the formation of the crescent membrane that eventually leads to the virion assembly. This cysteine protease is also responsible for the cleavage of several core proteins, including P25K/VP8, P4a, and P4b, which form more than 30% of the mass of the virion [1-2].

References

  1. Liu, L.; Cooper, T.; Howley, P.M.; Hayball, J.D. From Crescent to Mature Virion: Vaccinia Virus Assembly and Maturation. Viruses. 2014, 6, 3787.
  2. Byrd, C.M.; Hruby, D.E. Vaccinia Virus Proteolysis—A Review. Rev. Med. Virol. 2006, 16, 187–202.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 563
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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