Coding
Part:BBa_K4843001
Designed by: Wu Delu Group: iGEM23_TSB-Center (2023-08-07)
ASFV-I7L gene sequence
Basic Part: BBa_K4843001 (ASFV-i7L)
Profile
- Name: ASFV-i7L
- Base Pairs: 720 bp
- Origin: African swine fever virus
- Properties: The viral protease I7L, which is responsible for the cleavage of A17, a structural protein involved in the formation of the crescent membrane that eventually leads to the virion assembly.
Usage and Biology
According to the literature, within the replication cycle of the model vaccinia virus, a fundamental step is the maturation of non-infective virions from immature intracellular virions (IV) to mature intracellular virions (IMV). This maturation process is linked to the activity of the viral protease I7L, which is responsible for the cleavage of A17, a structural protein involved in the formation of the crescent membrane that eventually leads to the virion assembly. This cysteine protease is also responsible for the cleavage of several core proteins, including P25K/VP8, P4a, and P4b, which form more than 30% of the mass of the virion [1-2].
References
- Liu, L.; Cooper, T.; Howley, P.M.; Hayball, J.D. From Crescent to Mature Virion: Vaccinia Virus Assembly and Maturation. Viruses. 2014, 6, 3787.
- Byrd, C.M.; Hruby, D.E. Vaccinia Virus Proteolysis—A Review. Rev. Med. Virol. 2006, 16, 187–202.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 563
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |