Plasmid_Backbone

Part:BBa_K4841017

Designed by: Qiu Zexuan   Group: iGEM23_TSBC-SZ   (2023-08-12)


pCOLA-PesaS-RBS-RFP-rrnbT1-CI857-PR-SRRz-rrnB

pCOLA-PesaS-RBS-RFP-rrnbT1-CI857-PR-SSRz-rrnB is the plasmid backbone for heat-induced autolysis.CI857 acts as a heat shock promoter to promote the expression of SRRz lysis genes.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 4111
    Illegal NheI site found at 5340
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 206
    Illegal BamHI site found at 954
    Illegal XhoI site found at 2154
    Illegal XhoI site found at 4006
    Illegal XhoI site found at 4117
    Illegal XhoI site found at 5989
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4325


[edit]
Categories
Parameters
None