Plasmid_Backbone

Part:BBa_K4841016

Designed by: Qiu Zexuan   Group: iGEM23_TSBC-SZ   (2023-08-12)


pACYC-pJ23117-esaR(I70V)-rrnBT -LSPT

pACYC-pJ23117-esaR(I70V)-rrnBT -LSPT


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2238
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 583
    Illegal NheI site found at 1421
    Illegal NheI site found at 1444
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 1471
    Illegal BamHI site found at 2492
    Illegal XhoI site found at 2565
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2238
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2238
    Illegal NgoMIV site found at 2823
    Illegal NgoMIV site found at 2983
    Illegal NgoMIV site found at 3337
    Illegal NgoMIV site found at 3917
    Illegal AgeI site found at 669
    Illegal AgeI site found at 993
    Illegal AgeI site found at 2089
    Illegal AgeI site found at 3741
    Illegal AgeI site found at 4201
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None