Coding

Part:BBa_K4841008

Designed by: Wang Zhitong   Group: iGEM23_TSBC-SZ   (2023-08-12)


esaRI70V

is a mutant version of the wild-type esaR that was previously engineered to respond to concentrations of AHL at a higher threshold than the wild-type protein.
binds the PesaS promoter in the absence of 3-oxohexanoylhomoserine lactone (AHL) and acti vates transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 613
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None