Coding

Part:BBa_K4835021

Designed by: Yue Ke   Group: iGEM23_XJU-China   (2023-10-11)


sufB is an iron-sulfur cluster scaffolding complex subunit, and iron-sulfur clusters may play an imp

sufB is a complex subunit of iron-sulfur cluster scaffolding, and iron-sulfur clusters are widely found in various organisms and are involved in important functions such as electron transfer, substrate binding and activation, and gene expression and regulation, etc. This gene may be useful for the improvement of a variety of microorganisms, especially for enhancing salinity tolerance in model organisms.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 625
    Illegal PstI site found at 1405
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 625
    Illegal PstI site found at 1405
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 497
    Illegal BamHI site found at 580
    Illegal XhoI site found at 439
    Illegal XhoI site found at 1300
    Illegal XhoI site found at 1381
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 625
    Illegal PstI site found at 1405
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 625
    Illegal PstI site found at 1405
    Illegal NgoMIV site found at 382
    Illegal NgoMIV site found at 532
    Illegal NgoMIV site found at 1093
    Illegal AgeI site found at 475
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 478
    Illegal BsaI.rc site found at 1306
    Illegal SapI site found at 198
    Illegal SapI site found at 1275


[edit]
Categories
Parameters
None