Coding

Part:BBa_K4831002

Designed by: Joakim Gustafsson   Group: iGEM23_ABOA-Turku   (2023-10-01)


MerB alkylmercury lyase from E. coli (seq. harmonized for Synechocystis sp. PCC6803)

Overview

MerB protein is an Organomercurial lyase that cleaves the bond between C-Hg and releases Hg(II) in addition to the appropriate organic compound. It can be used as a combination with merA, which is a mercuric reductase. Together or alone, merB can be used to increase the resistance to organic mercury (methyl mercury) or mercuric compounds like mercuric (II)chloride. It can be used as a tool for bioremediation.

Design Notes

The sequences codons have been harmonized for optimal translation in Synechocystis sp. PCC 6803 (127 changed codons, GC-change 56.6 to 51.1%). NOTE! starting codon ATG has been removed to be compatible with our composite part (reading frame shift).

Source

The part is from Escherichia coli plasmid pAR060302, complete sequence. https://www.ncbi.nlm.nih.gov/nuccore/NC_012692.1?report=genbank&from=134760&to=135398&strand=true Aminoacid sequence: https://www.uniprot.org/uniprotkb/P77072/entry


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 128
    Illegal XbaI site found at 109
    Illegal XbaI site found at 622
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 128
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 128
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 128
    Illegal XbaI site found at 109
    Illegal XbaI site found at 622
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 128
    Illegal XbaI site found at 109
    Illegal XbaI site found at 622
  • 1000
    COMPATIBLE WITH RFC[1000]


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