Part:BBa_K4831000

MerA (mercuric reductase) from Synechocystis sp. PCC6803

Overview

MerA protein catalyzes the reduction of mercuric ion (Hg2+) to elemental mercury. The merA -gene codes for mercuric reductase (EC 1.16.1.1) that catalyzes the reduction of toxic ionic mercury (Hg2+) to less volatile and toxic elemental mercury (Hg0). It can be used as a combination with merB, which is a organomercury lyase. Together or alone, merA can be used to increase the resistance to organic mercury (methyl mercury) or mercuric compounds like mercuric (II)chloride. It can be used as a tool for bioremediation.

Design Notes

This part is from Synechocystis sp PCC6803. One codon was changed to avoid illegal restriction sites for BsaI or SapI. NOTE! starting codon ATG has been removed to be compatible with our composite part (reading frame shift).

Source

The part is from Synechocystis sp. PCC6803 genomic sequence.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]