Part:BBa_K4829018
IVT of this sequence produces mRNA coding for the CD36 membrane protein
This composite biobrick is one of multiple combinations possible using our modular mRNA-based protein expression systems. We have not produced this using IVT. However, considering our success with BBa_K4829003, we can confidently say that this will produce the required membrane protein. We would like to caution users of this sequence here, however, as this is a GLYCOSYLATED protein, and though literature suggests that this is not a problem, glycosylation is a cell-type DEPENDENT process, and the extent of glycosylation would vary from cell type to cell type.
Usage and Biology
CD36 (or SR-B3) is a cell surface receptor expressed on macrophages, among other cell types. It belongs to the class B scavenger receptor family and is crucial in lipid metabolism and immune responses; SR-B3 on macrophages functions as a multifunctional receptor involved in recognising, binding, and internalising a wide range of ligands, including modified lipoproteins, apoptotic cells, microbial pathogens, and various fatty acids. By mediating the uptake of these diverse ligands, SR-B3 contributes to the clearance of apoptotic cells and cellular debris, regulating lipid homeostasis and modulating inflammatory responses. Clearly, SR-B3 on macrophages play a pivotal role in immune responses. What is more important in our context, however, is that macrophages appear to lose their phagocytic ability when CD36 is downregulated. This has been proposed to occur because of higher concentrations of PGE2 in the peritoneal fluid of women with endometriosis (which has been shown to exist).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 527
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 165
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1349
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Figure 1: The structure of the RNA of the CDS produced on IVT of this sequence. 'Loss' function value (AUP) = 482.1750000000001
Scale of the AUP
For more information on the AUP, please check out out pages of BBa_K4829019 onwards
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