Part:BBa_K4829004
5' UTR patented by Modernatx
This 5'UTR is derived from the beta-globin UTR's, and has been modified by Modernatx to produce non-human proteins in human cells. Please find more details in Patent number US10925935B2
Usage and Biology
5' UTRs: Untranslated Regions at the mRNA's Beginning
The 5' UTR, or 5' Untranslated Region, is a crucial component found at the beginning of a messenger RNA (mRNA) molecule. This region, though not coding for proteins itself, holds significant importance in the world of genetics and gene expression. Here's why 5' UTRs are essential:
- Translation Initiation: The 5' UTR contains vital elements that govern the initiation of translation, the process where ribosomes start synthesizing proteins from the mRNA. Key elements within the 5' UTR, such as the Kozak sequence and the 5' cap structure, are essential for ribosome binding and the accurate start of protein synthesis.
- Regulatory Functions: Beyond its role in translation initiation, the 5' UTR also houses regulatory elements that control gene expression. These elements include binding sites for proteins and RNA-binding molecules. By interacting with these elements, the 5' UTR can modulate the rate at which the mRNA is translated into protein. This regulation is crucial for finely tuning protein production in response to cellular needs.
5' UTR of mRNA-1273:
- The Moderna vaccine mRNA incorporates the 5' UTR of a human β-globin gene.
- Rationale: The choice to use the 5' UTR of the human β-globin gene is attributed to its efficient translation properties. The β-globin mRNAs are known to be translated very efficiently, making them a prime candidate for ensuring effective protein production from the vaccine mRNA inside human cells.
The 5' UTR plays a crucial role in mRNA stability and the initiation of translation, ensuring that the mRNA is correctly recognized by the cellular machinery and effectively translated into protein.
In academia, where using patented sequences is permitted, as long as the correct acknowledgement is given, one can use these sequences as seen in our BBa K4829003. This is done when the requirement to optimise or profit out of the mRNA is not required. We would like to remind all users, once again, that using these sequences without acknowledgement or using these sequences for making a profit, is STRICTLY PROHIBITED.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
We have used this part in BBa K4829003, and we noticed expression in Hela cells, 6hrs post-transfection.
Figure 1. Expression of the protein noted in the supernatant 6 hours post transfection
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