Primer

Part:BBa_K4816005

Designed by: Julia Alexander   Group: iGEM23_Bonn-Rheinbach   (2023-09-25)

norB primer forward

  • Primer design

This primer was designed with a region to flank the 5' terminus of the gene NorB in Paraccocus denitrificans PD1222 part of the chromosome 1 (NCBI ID:CP000489.1) located between base pairs (2498986 and 2500374). The primer contains the addition of the restriction site NdeI and 6 bases to allow the cut of the restriction endonuclease. The introduction of the unique restriction site NdeI into the gene NorB allows the precise introduction of NorB in the plasmid vector pet 23 a (+) for protein expression and further purification. The primer contains a 19 bp DNA binding region. 

 CAGTCACATATGATGAGATATCATTCGCAAC
       ******-------------------
        NdeI  DNA binding region

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None