Composite
Part:BBa_K4814002
Designed by: CHIEN-YUEH LIU Group: iGEM23_PuiChing-Macau (2023-09-28)
RecA(K3)-B0032-eGFP
To optimize the design of the RecA-eGFP reporter (BBa_K4814015), we replaced the original B0034 RBS with RBS BBa_B0032, and see if the effectiveness will increase. However, from our experimental result, using RBS BBa_K4814002 does not enhance the level of fluorescence of the reporter.
RBS test of UVB and H2O2 FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.
RBS test of Nalidixic acid and Aspartame FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 882
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 840
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |