Regulatory
Part:BBa_K4813026
Designed by: Paul Lau Group: iGEM23_HongKong-JSS (2023-10-09)
Reverse J23100 strong constitutive promoter
This part encodes the reverse sequence of the J23100 promoter, which is a well-known strong constitutive promoter from the Anderson promoter collection. We incorporate this promoter in our construct along with a native HxlR operon, which is a formaldehyde-inducible operon. This choice is made because the native expression unit of the HxlR protein is in the reverse direction, necessitating the use of the J23100 promoter to drive its expression effectively.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
Illegal NheI site found at 24 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |