Regulatory

Part:BBa_K4813026

Designed by: Paul Lau   Group: iGEM23_HongKong-JSS   (2023-10-09)

Reverse J23100 strong constitutive promoter

This part encodes the reverse sequence of the J23100 promoter, which is a well-known strong constitutive promoter from the Anderson promoter collection. We incorporate this promoter in our construct along with a native HxlR operon, which is a formaldehyde-inducible operon. This choice is made because the native expression unit of the HxlR protein is in the reverse direction, necessitating the use of the J23100 promoter to drive its expression effectively.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NheI site found at 24
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None