Part:BBa_K4813020

Forwardly transcribed HxlR K13A - mutated HxlR codon with enhanced DNA binding and transcription act

The coding sequence mentioned encodes a protein called HxlR, which is a formaldehyde-responsive transcription factor from Bacillus subtilis. According to research, when the 13th amino acid in HxlR protein, Lysine, was replaced by Alanine, mutated HxlR-K13A protein showed a more optimal conformation for DNA binding activity and a higher transcription activation than the wild-type HxlR protein [1].

In our project, we would like to see if a forwardly transcribed HxlR can still be expressed and retain its function. Therefore, besides having HxlR forwardly transcribed in our constructs, we also design to have HxlR-K13A forwardly transcribed under a forward promoter.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]