RBS
Part:BBa_K4813019
Designed by: Chong Tsz Ting, Jason Group: iGEM23_HongKong-JSS (2023-10-08)
Modified RBS for T7 promoter
The RBS in pET28a(+)-FGF2 is modified to increase gene expression and product yield [1]. We use this modified RBS in our construct to enhance the expression of our gene of interest.
References
[1]Shilling, P. J., Mirzadeh, K., Cumming, A. J., Widesheim, M., Köck, Z., & Daley, D. O. (2020). Improved designs for pet expression plasmids increase protein production yield in escherichia coli. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-0939-8
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |