Part:BBa_K4813016

BRH1 and BRH2 - wild type HxlR protein binding site

The HxlR protein is a formaldehyde-responsive transcription factor from Bacillus subtilis located upstream of the hxlAB operon [1,2]. There are two necessary DNA binding sites (BRH1&2) for HxlR to bind to and subsequently activate the expression of the downstream hxlAB operon in the presence of formaldehyde [1].

References

[1]Yurimoto, Hiroya, et al. “HXLR, a member of the duf24 protein family, is a DNA-binding protein that acts as a positive regulator of the formaldehyde-induciblehxlaboperon inbacillus subtilis.” Molecular Microbiology, vol. 57, no. 2, 2005, pp. 511–519, https://doi.org/10.1111/j.1365-2958.2005.04702.x

[2]Zhu, Rongfeng, et al. “Genetically encoded formaldehyde sensors inspired by a protein intra-helical crosslinking reaction.” Nature Communications, vol. 12, no. 1, 2021, https://doi.org/10.1038/s41467-020-20754-4.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]