Reporter

Part:BBa_K4811003

Designed by: Kasper Krunderup Jakobsen   Group: iGEM23_DTU-Denmark   (2023-09-08)


pBAD-BRA-mCherry

mCherry under control by the pBAD promoter, along with the repressible ribosome binding site (RBS), BRA. This means that mCherry transcription is positively regulated by L-arabinose, and mCherry translation is negatively controlled by the tRNA mimicking structure (TMS) BBa_K4811000, and related parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None