Primer

Part:BBa_K4807014

Designed by: Vaios Styliaras   Group: iGEM23_IOANNINA   (2023-10-11)


Reverse Primer for hemA(s) insert

Primer designed for the integration of hemA(s) gene into pET-29c(+). This primer consists of a 20-base overlap region from pET-29c(+) and a 15-base gene-specific region from the hemA(s) insert. It also shares the same overlap region with the Forward Primer for hemF insert (BBa_K4807015) in order to attach these two inserts and the hemL insert into one united insert.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None