Primer

Part:BBa_K4807009

Designed by: Vaios Styliaras   Group: iGEM23_IOANNINA   (2023-10-11)


Forward Primer for rhtA insert

Primer designed for the integration of rhtA gene into pETDuet-1. This primer consists of a 20-base overlap region from pETDuet-1 and a 15-base gene-specific region from the rhtA insert.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 14
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 14
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 14
    Illegal BamHI site found at 8
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 14
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None