Primer

Part:BBa_K4807008

Designed by: Vaios Styliaras   Group: iGEM23_IOANNINA   (2023-10-11)


Reverse Primer for CAT insert

Primer designed for the integration of hemD gene into pETDuet-1. This primer consists of a 20-base overlap region from pETDuet-1 and a 15-base gene-specific region from the CAT insert.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None