Protein_Domain

Part:BBa_K4803014

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


KKYL (ER retention signal)

Usage and Biology

The four amino acid sequence of K-K-Y-L at the N-terminus induces the secretory protein to remain in the endoplasmic reticulum [1].

In UTokyo2023 project, this part was used to design the Secretion system. In this system, proteins are retained in the ER by retention signal until the signal is excised by proteases.

control
Figure 1. Overview of Secretion system

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

[1] Vlahos, A. E., Kang, J., Aldrete, C. A., Zhu, R., Chong, L. S., Elowitz, M. B., & Gao, X. J. (2022). Protease-controlled secretion and display of intercellular signals. Nature communications, 13(1), 912.


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