Protein_Domain

Part:BBa_K4803012

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


3xFCS (furin cleavage site)

Furin Cleavage Site (FCS) is an amino acid sequence that is cleaved by Furin.

This part is stacked with three FCS so that cleavage by furin occurs more reliably.

This sequence was designed based on the paper in [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

[1] Vlahos, A. E., Kang, J., Aldrete, C. A., Zhu, R., Chong, L. S., Elowitz, M. B., & Gao, X. J. (2022). Protease-controlled secretion and display of intercellular signals. Nature communications, 13(1), 912.



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