Part:BBa_K4803011

TRE3G promoter

Usage and Biology

TRE3G promoter is the third generation of Tet system found by Zhou et al. (2006). It has seven-fold more active and 100-fold more dox-sensitive than the original Tet-On system [1]. This is applicable both to tet-on system to tet-off system. Same as the former version, the presence of tetracycline or doxycycline regulates the Tetracycline Response Element (TRE) and downstream promoter expression [2].

In UTokyo2023 project, we used the tet-off system in the project.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Zhou, X., Vink, M., Klaver, B., Berkhout, B., & Das, A. T. (2006). Optimization of the tet-on system for regulated gene expression through viral evolution. Gene Therapy, 13(19), 1382-1390. https://doi.org/10.1038/sj.gt.3302780

[2] Osumi, N. (2012, July 24). Tet-on/off Systems. Brain Science Dictionary. https://bsd.neuroinf.jp/wiki/Tet_on/off%E3%82%B7%E3%82%B9%E3%83%86%E3%83%A0