Regulatory

Part:BBa_K4803010

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


mini promoter

This promoter was constructed based on the paper in [1] as a promoter regulated by STAT3 responsive element/AP-1 responsive element.

Without the responsive sequence, this sequence acts as a negative control promoter.

In UTokyo2023 this part was combined with STAT3 responsive element for IL-6 reporter and with AP-1 responsive element for IL-1 reporter used as a promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 27
    Illegal BamHI site found at 13
    Illegal XhoI site found at 23
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1]Wei, W., Tweardy, D. J., Zhang, M., Zhang, X., Landua, J., Petrovic, I., Bu, W., Roarty, K., Hilsenbeck, S. G., Rosen, J. M., & Lewis, M. T. (2014). STAT3 signaling is activated preferentially in Tumor‐Initiating cells in Claudin‐Low models of human breast cancer. Stem Cells (Dayton, Ohio), 32(10), 2571-2582. https://doi.org/10.1002/stem.1752



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