Part:BBa_K4803008

AP-1 responsive element

Usage and Biology

AP-1 signaling pathway works in response to cytokines. This part activates the downstream promoter by binding to the AP-1 protein.

In UTokyo2023 project, we needed to confirm the function of the MESA system [1] to detect IL-1β and whether the concentration of IL-1β is sufficiently altered by inhibitors secreted by the Secretion unit. Therefore, we thought it would be possible to quantify the concentration of IL-1β by monitoring AP-1.

For the sequence itself, we referred to a paper [2].

Figure 1. The Signaling Pathway of IL-1β[3].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Daringer, N. M., Dudek, R. M., Schwarz, K. A., & Leonard, J. N. (2014). Modular extracellular sensor architecture for engineering mammalian cell-based devices. ACS synthetic biology, 3(12), 892-902. https://doi.org/10.1021/sb400128g

[2] Jutz, S., Leitner, J., Schmetterer, K., Doel-Perez, I., Majdic, O., Grabmeier-Pfistershammer, K., Paster, W., Huppa, J. B., & Steinberger, P. (2016). Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-κB, NFAT and AP-1. Journal of Immunological Methods, 430, 10-20. https://doi.org/10.1016/j.jim.2016.01.007

[3] Invivogen. (n.d.). IL-1β Reporter HEK 293 Cells. Products, InvivoGen. https://www.invivogen.com/hek-blue-il6