Reporter

Part:BBa_K4799604

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pNtNRAMP5_GUS_T-Nos

pNtNRAMP5 is a promoter. It is added before the reporter gene β-Glucuronidase (gus) and NOS terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1027
    Illegal SpeI site found at 1391
    Illegal SpeI site found at 1921
    Illegal SpeI site found at 2240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4049
    Illegal SpeI site found at 1027
    Illegal SpeI site found at 1391
    Illegal SpeI site found at 1921
    Illegal SpeI site found at 2240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2031
    Illegal BamHI site found at 2014
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1027
    Illegal SpeI site found at 1391
    Illegal SpeI site found at 1921
    Illegal SpeI site found at 2240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1027
    Illegal SpeI site found at 1391
    Illegal SpeI site found at 1921
    Illegal SpeI site found at 2240
    Illegal NgoMIV site found at 2464
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1486
    Illegal BsaI.rc site found at 2264
    Illegal BsaI.rc site found at 2879
    Illegal SapI.rc site found at 3050


[edit]
Categories
Parameters
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