Tag

Part:BBa_K4790077

Designed by: Hongyan Jing   Group: iGEM23_NNU-CHINA   (2023-10-11)


6xHis

The 6xHis tag is utilized for affinity purification of proteins. This fusion tag can bind to Ni2+-affinity resin through coordination between Ni2+ ions and histidine residues. It allows purification of the protein of interest carrying the 6xHis tag after washing and elution steps.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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