![](https://parts.igem.org/images/partbypart/icon_tag.png)
Tag
Part:BBa_K4790077
Designed by: Hongyan Jing Group: iGEM23_NNU-CHINA (2023-10-11)
6xHis
The 6xHis tag is utilized for affinity purification of proteins. This fusion tag can bind to Ni2+-affinity resin through coordination between Ni2+ ions and histidine residues. It allows purification of the protein of interest carrying the 6xHis tag after washing and elution steps.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |