Coding

Part:BBa_K4790071

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-09-28)


ASR6(node 112)

Node 112 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node 112, and find variants with excellent properties.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 281
    Illegal PstI site found at 292
    Illegal PstI site found at 418
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 268
    Illegal PstI site found at 281
    Illegal PstI site found at 292
    Illegal PstI site found at 418
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 288
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 281
    Illegal PstI site found at 292
    Illegal PstI site found at 418
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 281
    Illegal PstI site found at 292
    Illegal PstI site found at 418
    Illegal NgoMIV site found at 264
  • 1000
    COMPATIBLE WITH RFC[1000]


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