Coding

Part:BBa_K4790070

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-09-28)


ASR5(node 111)

Node 111 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node 111, and find variants with excellent properties.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 286
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 205
    Illegal PstI site found at 286
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 286
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]


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