Coding

Part:BBa_K4790069

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-09-28)


ASR4(node 109)

Node 109 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node 109, and find variants with excellent properties.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 289
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 289
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 289
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 289
  • 1000
    COMPATIBLE WITH RFC[1000]


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