Plasmid

Part:BBa_K4790067

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-09-26)


PETase-node59

The vector is constructed based on pET-22b. We amplified pET-22b by PCR and use Gibson Assembly method. DNA fragment node 59 was subcloned into pET-22b vector between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is ampicillin resistance gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5297
    Illegal EcoRI site found at 6114
    Illegal XbaI site found at 5163
    Illegal PstI site found at 1140
    Illegal PstI site found at 5415
    Illegal PstI site found at 5547
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5297
    Illegal EcoRI site found at 6114
    Illegal NheI site found at 6036
    Illegal PstI site found at 1140
    Illegal PstI site found at 5415
    Illegal PstI site found at 5547
    Illegal NotI site found at 6234
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5297
    Illegal EcoRI site found at 6114
    Illegal BglII site found at 5097
    Illegal BamHI site found at 5291
    Illegal XhoI site found at 6243
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5297
    Illegal EcoRI site found at 6114
    Illegal XbaI site found at 5163
    Illegal PstI site found at 1140
    Illegal PstI site found at 5415
    Illegal PstI site found at 5547
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5297
    Illegal EcoRI site found at 6114
    Illegal XbaI site found at 5163
    Illegal PstI site found at 1140
    Illegal PstI site found at 5415
    Illegal PstI site found at 5547
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3317
    Illegal NgoMIV site found at 3477
    Illegal NgoMIV site found at 5065
    Illegal NgoMIV site found at 5258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1314
    Illegal SapI.rc site found at 2396


[edit]
Categories
Parameters
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