Coding

Part:BBa_K4790064

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-08-13)


ASR1(node 59)

Node 59 is the ancestral sequence of PETase predicted by constructing a phylogenetic tree using specific algorithm based on some enzymes that have been reported to be capable of hydrolyzing plastics. That is to say, it is an ancient protein that we resurrected, and it is speculated to have excellent properties such as good temperature stability and mutation stability. In this project, we will use machine learning for prediction, mutate node 59, and find variants with excellent properties.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 799
    Illegal NheI site found at 721
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 799
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 1000
    COMPATIBLE WITH RFC[1000]


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