Plasmid

Part:BBa_K4790001

Designed by: Hongyan Jing   Group: iGEM23_NNU-CHINA   (2023-08-14)


pET-22b(+)

pET-22b(+) is a prokaryotic expression vector which was used to ligate the predicted ancestral sequences. After it was successfully ligated to each ancestral sequence, we introduced the ligated plasmid into E. coli for expression to obtain the target protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 650
    Illegal PstI site found at 4673
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4673
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 716
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 650
    Illegal PstI site found at 4673
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 650
    Illegal PstI site found at 4673
    Illegal NgoMIV site found at 555
    Illegal NgoMIV site found at 748
    Illegal NgoMIV site found at 2336
    Illegal NgoMIV site found at 2496
    Illegal NgoMIV site found at 5676
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 505
    Illegal BsaI.rc site found at 4499
    Illegal SapI site found at 3416


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