Coding

Part:BBa_K4786005

Designed by: Xiran Hu   Group: iGEM23_Tongji-China   (2023-10-10)


GmPilA mutant 3

GmPilA encodes the monomer that can assemble into type Ⅳ pilus in Geobacter metallireducens which is electric conductive.The type, number and relative position of the aromatic amino acids inside the pili change the conductivity of the pili. This variant of GmPilA has three mutation alleles which are A31F,Q55F and N59F.GmPilA mutant 1 was predicted to have higher conductivity than wild type pili of Geobacter metallireducens.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None