Coding

Part:BBa_K4782008

Designed by: Adina Kadyrova   Group: iGEM23_NU-Kazakhstan   (2023-10-12)


ALPaGA + Colicin E1 + double terminator

Origin: Escherichia coli, synthetic Properties: Colicin E1 expression induced by sodium lactate in anoxia and glucose rich environment Design: codon optimized LldRPD promoter for glucose-rich and anoxic environment Subparts: BBa_K4244000, BBa_K2922024


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1535
    Illegal AgeI site found at 1592
    Illegal AgeI site found at 1724
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None