Coding

Part:BBa_K4780012

Designed by: Jack Dalton   Group: iGEM23_ANU-Australia   (2023-10-11)


RFD Ar Binding 2.3

Synthetic arsenic binding protein designed using RFDiffusion neural network. We had difficulties with DNA synthesis purity and cloning due to high GC content.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 274
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 274
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 274
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 274
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 124


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Categories
Parameters
None