Coding

Part:BBa_K4780007

Designed by: Jack Dalton   Group: iGEM23_ANU-Australia   (2023-10-11)


ArsR MPNN 8

This is a synthetic Arsenic binding protein based on E. coli ArsR designed using protein MPNN. Did not express well in E. coli DE3 cells. We switched to T7 Shuffle cells as they express cysteine rich proteins better and this resulted in strong expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 187
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 187
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 187
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 187
    Illegal NgoMIV site found at 175
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None