Coding

Part:BBa_K4780006

Designed by: Jack Dalton   Group: iGEM23_ANU-Australia   (2023-10-11)


ArsR MPNN 7

This is a synthetic Arsenic binding protein based on E. coli ArsR designed using protein MPNN. Did not express well in E. coli DE3 cells. We switched to T7 Shuffle cells as they express cysteine rich proteins better and this resulted in strong expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 197
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 197
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 197
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 197
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None