Coding
Part:BBa_K4780005
Designed by: Jack Dalton Group: iGEM23_ANU-Australia (2023-10-11)
ArsR MPNN 6
This is a synthetic Arsenic binding protein based on E. coli ArsR designed using protein MPNN. Did not express well in E. coli DE3 cells. We switched to T7 Shuffle cells as they express cysteine rich proteins better and this resulted in strong expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |