Composite

Part:BBa_K4773019

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-02)


Lac Promoter+RBS+HAO+RBS+HmpA+Term

This part is a pathway combination consisting of HAO and HmpA, which can degrade ammonia (NH3), a common nitrogenous waste, to nitrate (NO3-) and nitric oxide (NO) in aerobic bacteria. HAO is a hydroxylamine oxidoreductase from Nitrosomonas europaea, which can oxidize hydroxylamine (NH2OH), a chemical produced biologically by the enzyme ammonia monooxygenase, to nitric oxide and water. HmpA is a flavohemoglobin from Escherichia coli, which can reduce nitric oxide to nitrate using NADH and oxygen. The part also contains a strong bacterial ribosome binding site (RBS) (BBa_B0034) before each gene to ensure efficient translation. A double terminator (BBa_B0015) is added at the end of the part to prevent read-through transcription and ensure the stability of the mRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1586
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None