Composite

Part:BBa_K4773014

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-02)


J23100 Promoter+RBS+hps+RBS+phi+Term

This part is a gene combination consisting of hps and phi, which can degrade formaldehyde, a toxic and carcinogenic compound, to formate and hydrogen peroxide. hps is a hexulose-6-phosphate synthase from Bacillus subtilis, which can condense formaldehyde and ribulose-5-phosphate to form hexulose-6-phosphate. phi is a phosphohexuloisomerase from Bacillus subtilis, which can isomerize hexulose-6-phosphate to fructose-6-phosphate. The part also contains a strong bacterial ribosome binding site (RBS) (BBa_B0034) before each gene to ensure efficient translation. A double terminator (BBa_B0015) is added at the end of the part to prevent read-through transcription and ensure the stability of the mRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 430


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Categories
Parameters
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