Composite

Part:BBa_K4773013

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-01)


J23100+RBS+ycnE+RBS+FMO+Term

This part is a gene combination consisting of ycnE and FMO, which can degrade indole, a heterocyclic aromatic compound, to different colored pigments. ycnE is a monooxygenase from Escherichia coli, which can oxidize indole to indigo, a blue dye. FMO is a flavin-containing monooxygenase from Pseudomonas putida, which can hydroxylate indole to indirubin, a purple-red dye. The part also contains a strong bacterial ribosome binding site (RBS) (BBa_B0034) before each gene to ensure efficient translation. A double terminator (BBa_B0015) is added at the end of the part to prevent read-through transcription and ensure the stability of the mRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None