Composite

Part:BBa_K4773012

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-01)


Lac Promoter+RBS+SQR+RBS+SDO+RBS+AprBA+RBS+SAT+Term

This part is a pathway combination consisting of SQR, SDO, AprBA, and SAT, which can degrade hydrogen sulfide (H2S), a toxic and malodorous gas, to sulfate (SO42-) and adenosine 5’-phosphosulfate (APS) in aerobic bacteria. SQR is a sulfide:quinone oxidoreductase from Acidithiobacillus thiooxidans, which can oxidize H2S to elemental sulfur (S0) and transfer electrons to quinone. SDO is a sulfur dioxygenase from Aquifex aeolicus, which can convert S0 to sulfite (SO32-) using molecular oxygen. AprBA is a dissimilatory adenosine 5’-phosphosulfate reductase from Desulfovibrio vulgaris, which can reduce APS to sulfite and AMP in sulfate-reducing bacteria. SAT is a sulfate adenylyl transferase from Escherichia coli, which can synthesize APS from sulfite and ATP. The part also contains a strong bacterial ribosome binding site (RBS) (BBa_B0034) before each gene to ensure efficient translation. A double terminator (BBa_B0015) is added at the end of the part to prevent read-through transcription and ensure the stability of the mRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4152
    Illegal SpeI site found at 2897
    Illegal PstI site found at 3698
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5122
    Illegal SpeI site found at 2897
    Illegal PstI site found at 3698
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3097
    Illegal BamHI site found at 4357
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4152
    Illegal SpeI site found at 2897
    Illegal PstI site found at 3698
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4152
    Illegal SpeI site found at 2897
    Illegal PstI site found at 3698
    Illegal NgoMIV site found at 1338
    Illegal NgoMIV site found at 1762
    Illegal NgoMIV site found at 2823
    Illegal NgoMIV site found at 4831
    Illegal NgoMIV site found at 5002
    Illegal NgoMIV site found at 5685
    Illegal AgeI site found at 2257
    Illegal AgeI site found at 2844
    Illegal AgeI site found at 3509
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None